BIOTRON LIMITED — Share Issue/Capital Change 2025

Dec 22, 2025

Appendix 2A - Application for quotation of securities

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Announcement Summary

Entity name

BIOTRON LIMITED

Announcement Type

New announcement

Date of this announcement

Tuesday December 23, 2025

The +securities to be quoted are:

+Securities issued, transferred or re-classified as a result of options being exercised or other +convertible securities being converted

Total number of +securities to be quoted

ASX +security		Number of +securities to
code	Security description	be quoted	Issue date
BITO	OPTION EXPIRING 24-NOV-2027	181,666,666	19/12/2025

Refer to next page for full details of the announcement

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Appendix 2A - Application for quotation of securities

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Part 1 - Entity and announcement details

1.1 Name of entity

BIOTRON LIMITED

We (the entity named above) apply for +quotation of the following +securities and agree to the matters set out in Appendix 2A of the ASX Listing Rules.

1.2 Registered number type

ABN

Registration number

60086399144

1.3 ASX issuer code

BIT

1.4 The announcement is

New announcement

1.5 Date of this announcement

23/12/2025

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Appendix 2A - Application for quotation of securities

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Part 2 - Type of Issue

2.1 The +securities to be quoted are:

+Securities issued, transferred or re-classified as a result of options being exercised or other +convertible securities being converted

2.2 The +class of +securities to be quoted is:

Additional +securities in a class that is already quoted on ASX ("existing class")

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Appendix 2A - Application for quotation of securities

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Part 3B - number and type of +securities to be quoted (existing class) where issue has not previously been notified to ASX in an Appendix 3B

Existing +securities converting into additional +securities in an existing class FROM (Existing Class) ASX +security code and description BITAK : OPTION EXPIRING 24-NOV-2027 EX $0.02

TO (Existing Class) ASX +security code and description BITO : OPTION EXPIRING 24-NOV-2027

Please state the number of options The first date the options were The last date the options were that were exercised or other exercised or other +convertible exercised or other +convertible +convertible securities that were securities were converted securities were converted converted 19/12/2025 19/12/2025 181,666,666 Is this all of the options or other +convertible securities on issue of that type (ie have all of those options now been exercised or have all of those convertible securities now been converted)? Yes The right of the holder of the options or other +convertible securities to receive the +underlying securities is being satisfied by: A reclassification of the +convertible securities as securities in the same class as the +underlying securities The underlying +securities being received by the holder are: Intended to be, but are not yet, quoted by ASX Were the options being exercised or other +convertible securities being converted issued under an +employee incentive scheme? No

Issue date

19/12/2025

Will the +securities to be quoted rank equally in all respects from their issue date with the existing issued +securities in that class? Yes

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Appendix 2A - Application for quotation of securities

Issue details

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Number of +securities to be quoted 181,666,666 Are the +securities being issued for a cash consideration? No Please describe the consideration being provided for the +securities Conversion of unquoted options to quoted options Please provide an estimate (in AUD) of the value of the consideration being provided per +security for the +securities to be quoted 0.000000 Any other information the entity wishes to provide about the +securities to be quoted No

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Appendix 2A - Application for quotation of securities

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Part 4 - Issued capital following quotation

Following the quotation of the +securities the subject of this application, the issued capital of the entity will comprise:

The figures in parts 4.1 and 4.2 below are automatically generated and may not reflect the entity's current issued capital if other Appendix 2A, Appendix 3G or Appendix 3H forms are currently with ASX for processing.

4.1 Quoted +securities (total number of each +class of +securities quoted on ASX following the +quotation of the +securities subject of this application)

+securities subject of this application)
	Total number of
ASX +security code and description	+securities on issue
BIT : ORDINARY FULLY PAID	2,409,199,260
BITO : OPTION EXPIRING 24-NOV-2027	289,310,011

4.2 Unquoted +securities (total number of each +class of +securities issued but not quoted on ASX)

	Total number of
ASX +security code and description	+securities on issue
BITAF : PERFORMANCE SHARES	500,000
BITAK : OPTION EXPIRING 24-NOV-2027 EX $0.02	0

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