bioAffinity Technologies, Inc. — Major Shareholding Notification 2025

Oct 2, 2025

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SCHEDULE 13G Field: Pseudo-Tag; ID: Name; Data: BIGGER CAPITAL FUND L P 0001288478 XXXXXXXX LIVE Common Stock, $0.007 par value 09/29/2025 0001712762 bioAffinity Technologies, Inc. 09076W307 3300 NACOGDOCHES ROAD SUITE 216 SAN ANTONIO TX 78217 Rule 13d-1(c) BIGGER CAPITAL FUND L P a DE 0.00 58682.00 0.00 58682.00 58682.00 N 2.89 PN Bigger Capital Fund GP, LLC a DE 0.00 58682.00 0.00 58682.00 58682.00 N 2.89 OO District 2 Capital Fund LP a DE 0.00 58682.00 0.00 58682.00 58682.00 N 2.89 PN District 2 Capital LP a DE 0.00 58682.00 0.00 58682.00 58682.00 N 2.89 PN District 2 GP LLC a DE 0.00 58682.00 0.00 58682.00 58682.00 N 2.89 OO District 2 Holdings LLC a DE 0.00 58682.00 0.00 58682.00 58682.00 N 2.89 OO Bigger Michael a X1 0.00 117364.00 0.00 117364.00 117364.00 N 5.79 IN bioAffinity Technologies, Inc. 3300 NACOGDOCHES ROAD, SUITE 216, SAN ANTONIO, TEXAS 78217 Bigger Capital Fund, LP ("Bigger Capital") Bigger Capital Fund GP, LLC ("Bigger GP") District 2 Capital Fund LP ("District 2 CF") District 2 Capital LP ("District 2") District 2 GP LLC ("District 2 GP") District 2 Holdings LLC ("District 2 Holdings") Michael Bigger Each of the foregoing is referred to as a "Reporting Person" and collectively as the "Reporting Persons." Bigger Capital Fund, LP 11700 West Charleston Blvd., #170-659 Las Vegas, NV, 89135 Bigger Capital Fund GP, LLC 11700 West Charleston Blvd., #170-659 Las Vegas, NV, 89135 District 2 Capital Fund LP 175 W. Carver Street Huntington, NY 11743 District 2 Capital LP 175 W. Carver Street Huntington, NY 11743 District 2 GP LLC 175 W. Carver Street Huntington, NY 11743 District 2 Holdings LLC 175 W. Carver Street Huntington, NY 11743 Michael Bigger 11700 West Charleston Blvd., #170-659 Las Vegas, NV, 89135 Bigger Capital Fund, LP Delaware Bigger Capital Fund GP, LLC Delaware District 2 Capital Fund LP Delaware District 2 Capital LP Delaware District 2 GP LLC Delaware District 2 Holdings LLC Delaware Michael Bigger USA Y As of September 29, 2025, Bigger Capital beneficially owned 58,682 shares of Common Stock. The amount does not include 61,490 shares of Common Stock issuable upon exercise of Pre-Funded Warrants, which were subject to a 9.99% beneficial ownership limitation. Bigger GP, as the general partner of Bigger Capital, may be deemed to beneficially own the Issuer's securities described herein. As of September 29, 2025, District 2 CF beneficially owned 58,682 shares of Common Stock. The amount does not include 41,433 shares of Common Stock issuable upon exercise of Pre-Funded Warrants, which were subject to a 9.99% beneficial ownership limitation. District 2, as the investment manager of District 2 CF, may be deemed to beneficially own the Issuer's securities described herein beneficially owned by District 2 CF. District 2 GP, as the general partner of District 2 CF, may be deemed to beneficially own the Issuer's securities described herein beneficially owned by District 2 CF. District 2 Holdings, as the managing member of District 2 GP, may be deemed to beneficially own the Issuer's securities described herein beneficially owned by District 2 CF Mr. Bigger, as the managing member of Bigger GP and the managing member of District 2 Holdings, may be deemed to beneficially own the: (i) 58,682 shares of Common Stock beneficially owned by Bigger Capital, and (ii) 58,682 shares of Common Stock beneficially owned by District 2 CF. Does not include: (a) 61,490 shares of Common Stock issuable upon exercise of Pre-Funded Warrants, which were subject to a 9.99% beneficial ownership limitation owned by Bigger Capital, and (b) 41,433 shares of Common Stock issuable upon exercise of Pre-Funded Warrants, which were subject to a 9.99% beneficial ownership limitation owned by District 2 CF. The foregoing should not be construed in and of itself as an admission by any Reporting Person as to beneficial ownership of any shares of Common Stock owned by another Reporting Person. Each of Bigger GP and Mr. Bigger disclaims beneficial ownership of the shares of Common Stock beneficially owned by Bigger Capital. Each of District 2, District 2 GP, District 2 Holdings and Mr. Bigger disclaims beneficial ownership of the shares of Common Stock beneficially owned by District 2 CF. The filing of this statement shall not be construed as an admission that any such person or entity is the beneficial owner of any such securities. The following percentages are based on the aggregate of: (i) 1,854,785 shares of Common Stock outstanding immediately before the offering, and (ii) 173,627 share of Common Stock, the number of shares of Common Stock being offered (excluding the shares of Common Stock issuable upon exercise of the Pre-Funded Warrants), based upon the Issuer's Prospectus filed under Rule 424(b)(4) with the Securities and Exchange Commission on September 30, 2025. As of September 29, 2025, (i) each of Bigger Capital and Bigger GP may be deemed to beneficially own 2.89% of the outstanding shares of Common Stock, (ii) each of District 2 CF, District 2, District 2 GP and District Holdings may be deemed to beneficially own 2.89% of the outstanding shares of Common Stock, and (iii) Mr. Bigger may be deemed to beneficially own approximately 5.79% of the outstanding shares of Common Stock. Does not include: (a) 61,490 shares of Common Stock issuable upon exercise of Pre-Funded Warrants, which were subject to a 9.99% beneficial ownership limitation owned by Bigger Capital, and (b) 41,433 shares of Common Stock issuable upon exercise of Pre-Funded Warrants, which were subject to a 9.99% beneficial ownership limitation owned by District 2 CF. Regarding Item 5 below, after September 29, 2025, the Reporting Persons sold all of the Common Stock, including, all the Common Stock issuable upon the exercise of the Pre-Funded Warrants that they held and accordingly, the Reporting Persons as of September 30, 2025, are not the beneficial owners of at least 5.00% of the Issuer's Common Stock. See Cover Pages Items 5-9. See Cover Pages Items 5-9. See Cover Pages Items 5-9. See Cover Pages Items 5-9. N Y Y Y N See Exhibit 99.1. Y N By signing below I certify that, to the best of my knowledge and belief, the securities referred to above were not acquired and are not held for the purpose of or with the effect of changing or influencing the control of the issuer of the securities and were not acquired and are not held in connection with or as a participant in any transaction having that purpose or effect, other than activities solely in connection with a nomination under ?? 240.14a-11. 99.1 - Joint Filing Agreement BIGGER CAPITAL FUND L P /s/ Michael Bigger Michael Bigger, Managing Member of Bigger Capital Fund GP, LLC, its general partner 10/01/2025 Bigger Capital Fund GP, LLC /s/ Michael Bigger Michael Bigger, Managing Member 10/01/2025 District 2 Capital Fund LP /s/ Michael Bigger Michael Bigger, Managing Member of District 2 GP LLC, its general partner 10/01/2025 District 2 Capital LP /s/ Michael Bigger Michael Bigger, Managing Member 10/01/2025 District 2 GP LLC /s/ Michael Bigger Michael Bigger, Managing Member 10/01/2025 District 2 Holdings LLC /s/ Michael Bigger Michael Bigger, Managing Member 10/01/2025 Bigger Michael /s/ Michael Bigger Michael Bigger 10/01/2025